MSc Final Oral Examination: Colbey Templeman


Interested Members of the University Community are invited to attend the Final Oral Examination for the Degree of Master of Science of Colbey Templeman of the Department of Plant Agriculture

Date: Tuesday, June 11th
Time: 1:00 PM
Room 202, Crop Science Building

Thesis Title: Differential DNA Methylation of Seed Yield QTL in Intercontinental Mega-Environments in a Canadian x Chinese Soybean Population

Advisory Committee
Dr. Istvan Rajcan, Advisor
Dr. Duane Falk
Dr. Lewis Lukens

Examination Committee
Dr. Ralph Martin, Chair
Dr. Istvan Rajcan
Dr. Duane Falk
Dr. Milad Eskandari


DNA methylation is an epigenetic modification that can produce phenotypic changes that impact a plant’s adaptation to the environment. Sodium bisulfite sequencing was carried out on the genomic regions associated with seed yield in multiple (QTLU) and specific (QTLSP) mega-environments (ME). One QTL(Satt139) and one QTLSP (Satt063) were evaluated on source seed grown in three MEs (Canada, USA, and China in 2010) of four selected RILs (28, 47, 4 and 90) derived from OAC Millennium x Heinong 38, and the parents. The source seed was grown in two field locations (Woodstock and Elora, ON) in both 2012 and 2013. Demethylation was observed in RIL 47 (China) in the Satt063 (QTLSP) region when grown in Elora and Woodstock in 2013. Due to methodology limitations, further research on DNA methylation is warranted to determine its role in the expression of yield QTL across different ME.