Raizada Lab Protocols

Introductory Tutorials and Links to Online Resources

A. Arabidopsis resources and molecular techniques 1
B. Genetic techniques and mutant analysis
C. Molecular techniques II and resources
D. Plant genomics tutorial and resources
E. Plant proteomics tutorial and resources
F. Plant biochemistry/metabolomics tutorial and resources
G. Molecular, other software tools and online links

Lab Protocols

1. Luciferase imaging – Versarray 1300B Specs
2. Luciferase imaging – Science STKE protocol from JKZhu’s Lab
3. Luciferase imaging – review Plant Mol Biol Rep (2000) 18, 143a-t
4. Luciferase imaging – gene trapping, Arabidopsis, Plant J (2003) 35, 273-283
5. Luciferase imaging – simple explanation from Kevin Smith, undergrad thesis
6. Luciferase imaging – excellent chapter Versarray system in Lum Biotech book
7. Luciferase plant tissue extraction, single cell luminometer protocol – M.Raizada
8. Luciferase -extraction and quantification – K.Luehrsen and V.Walbot, Stanford
9. Luciferase – use of Berthold MicroLumatPlus 96 well luminometer – M.Raizada
10. Flx800TBI Fluorescence Reader – Quick Protocol – M.Raizada
11. Flx800TBI Fluorescence Reader – Chemiluminescence detection theory
12. Flx800TBI Fluorescence Reader - dsDNA quantification using picoGreen
13. Flx800TBI Fluorescence Reader – ssDNA quantification using oliGreen
14. Flx800TBI Fluorescence Reader – RNA quantification using riboGreen
15. Flx800TBI Fluorescence Reader – protein quantification overview
16. Flx800TBI Fluorescence Reader – protein quantification using OPA
17. Flx800TBI Fluorescence Reader – protein quantification using CBCBQ
18. Flx800TBI Fluorescence Reader – quantifying calcium
19. Flx800TBI Fluorescence Reader – GFP quantification notes
20. Flx800TBI Fluorescence Reader – GFP quantification protocol
21. Hoefer Fluorometer/Flx800TBI – dsDNA quantification using Hoescht 33258
22. Hoefer Fluorometer/Flx800TBI – enhanced dsDNA quantification Hoescht
23. Hoefer Fluorometer – GUS quantification, Stockinger Lab, Ohio State
24. Protein quantification, Bradford Assay,SpectraMax Plate Reader, M.Raizada
25a. Maize - RescueMu protocols, Plant Functional Genomics 2003, M.Raizada
25b. Maize - transformation –refer to Raizada Website for detailed protocols
26. Maize – biolistic bombardment, M.Raizada
27. Agarose gels, Stockinger Lab, Ohio State
28. E.coli – heat shock transformation (M.Raizada)
29. E.coli – glycerol freezer stocks, Stockinger Lab, Ohio State
30. E.coli – directed protein evolution protocols, F.H. Arnold Lab, Caltech
31. Bacteriophage M13 protocols, M.Raizada
32. Northern Blot P Buffer Protocol M.Raizada
33. Physcomitrella – Growth and transformation M.Raizada/Neil Ashton
34. Physcomitrella – Protocols, Didier Schaefer, Univ of Lausanne
35. Physcomitrella – Gene targeting, Didier Schaefer, Curr Opin Plant Biol 2001
36a. Arabidopsis – check for TAIR protocols: http://www.arabidopsis.org/index.jsp
36b. Arabidopsis - General growth and maintenance M.Raizada
37. Arabidopsis – seed sterilization, Steve Chatfield, Raizada Lab
38. Arabidopsis regeneration – cotyledon regeneration protocol M.Raizada
39. Arabidopsis regeneration – germination and regeneration media M.Raizada
40. Arabidopsis regeneration – regeneration score sheet, Raizada Lab
41. Arabidopsis regeneration – tissue-scanning protocol, Raizada Lab
42. Arabidopsis – Agrobacterium transformation, Stockinger Lab, Ohio State
43. Arabidopsis – Agrobacterium transformation, Andrew Bent, Plant J 1998
44. Arabidopsis – Agrobacterium competent cells/protocols, TAIR website
45. Arabidopsis – Agrobacterium high-throughput transformation S.Cutler
46. Arabidopsis – transformation, Basta selection on soil
47. Arabidopsis – inexpensive DNA isolation mapping Plant Methods 2005
48. Arabidopsis – ploidy analysis protocol, David Galbraith, Plant Methods 2005
49. Raizada Lab task checklist
50. E.coli - use of ssDNA/tRNA to increase transformation efficiency
51. Steve Chatfield’s Guide to Working with Arabidopsis
52. Steve Chatfield’s GUS histochemical protocol