Department of Plant Agriculture Tools for molecular biology and genetics




Protocols
	General Protocols Sites Double Twist (protocols and Methods Discussion Forums) Molecular Biology Protocols (protocols by subject from various labs around the world at Double Twist) Comprehensive Protocol Collection (from Dartmouth University) Molecular Biology Protocols (US Dept Commerce/NOAA/NMFS/NWFSC) BioMedNet (technical tips and research tools) National Diagnostics (various protocols) Common Protocols Company Addresses Laboratory Safety Media Buffers Phosphate Buffers Stock Solutions Antibiotics Culture Collection Collecting Cotton Tissue Tissue Lyophilization Genomic DNA Purification Cotton DNA Isolation Arabidopsis DNA Purification from Nuclei Plant DNA Extraction Protocol - Meyerowitz Laboratory Plant DNA Extraction Protocol - PMB Plant Nuclei DNA Extraction Protocol - DP Plant Mitochondrial DNA Extraction Protocol - DP Plant Chloroplast DNA Extraction Protocol - DP Isolation of Genomic DNA from Azorhizobium caulinodans ORS571 DNA Extraction from ORS571 DNA Extraction Procedure for Rhizobium spp. General DNA Extraction Procedure for Bacteria ReadyAmp™ Genomic DNA Purification System Quiagen Preps Lessons In Paranoia RNA Preparation Methods RNA Extraction from Cotton Plant RNA from Potato Isolation Of RNA From Bacteroids and Bacteria Transformation Competent Cells Transformation One-step Transformation of E. coli Transformation of Yeast - LiAc Method X-Gal Large Scale Isolation of Plasmid and Bacterial DNA Alkaline Lysis DNA Purifications from Bacteria High-Copy Plasmid Purfication Using The TL-100 Rotor Xanthomonas campestris Plasmid DNA Mini-Prep Plasmid Isolation - Medium Plasmid Isolation - Intermediate Plasmid Isolation - Large Modified Eckhardt Gel Procedure DE52 DEAE Cellulose Phage Lambda DNA Purification Maxi-Prep Phage Lambda DNA Purification Maxi-Prep TL-100 Phage Purification TL-100 Phage Rapid Purification Protocol P1 DNA preparation P1 DNA PEG precipitation Fungal DNA Isolation Single-Fly DNA Preps for PCR Cesium Chloride Gradients Restriction Enzyme Buffer Recipes Restriction Enzyme Buffer Mixes Restriction Enzyme Digest Agarose Gel Electrophoresis Molecular Weight Standards Methylene Blue DNA Staining Glassmilk DNA Purification DNA from Low Melting Temperature Agarose Precipitating DNA Quantifying DNA Dephosphorylating DNA PCR Cloning Blunt-end PCR Fragments TA Subloning of PCR Fragments PCR site-directed mutagenesis Subloning of PCR Fragments RT-PCR Differential Display-Reverse Transcription-PCR Optimized Welsh DDRT-PCR Protocol DDRT-PCR Eluting Bands Microplate Templates PCR Error Rates Differential cDNA Screening Procedures Southern Transfers Southern Transfer - Sponge Method Hybridizations Hybridization Conditions - Northerns Post Hybridization DNA Probes Radioactive Labelling of DNA Randon Primer Oligo-labelling of DNA Random Primer Method Spin Columns PCR Protocol PCR Amplification from Microbial Colonies RAPDs Extra Long PCR (Church Lab) Sequencing Protocols Exonuclease Protocols Misc. Mutagenesis Equilibrating Phenol Siliconizing Glassware Preparing Salmon Sperm DNA Cleaning Radiation Clean-up for Ethidium Bromide Removal of CsCl/EtBr Waste Arabidopsis Seed Collection Arabidopsis Transformation Arabidopsis Vacuum Infiltration Preparation of DNA from Single Rice Seedlings Isolation of RNA and DNA from Rice Seedling Mitochondria A simple screning method for transgenic rice tissue based on PCR Non-aqueous Method for Purification of Cytoplasmic DNA from Individual Rice Plants for RFLP Analysis Enhancement of Division Frequency of Protoplasts and Plant Regeneration in Rice Isolation of RNA and DNA from different rice tissues Improved histochemical staining for beta-D-glucuronidase activity in monocotyledonous plants A rapid, reliable method for preparing somatic chromosomes of rice cDNA Topics Misc. Methods (DUMP FILE) Misc. Stocks Tissue Culture Stocks Misc. Strains A. Thaliana Topics Preparation Of Nylon Filters For Dot-blot DNA Hybridization Xanthomonas campestris Tri-Parental Mating Protocol Agrobacterium-mediated Transformation of Cotton MOPS Minimal Medium for Xanthomonas campestris Xanthomodadin Extraction from Xanthomonas campestris Bacteroid Isolation Quick Test For Restriction Enzymes
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Protocols