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MBG*3100 Plant Genetics

UNDERGRADUATEReproduction in plants, genetic and cytogenetic structure of plants, nature of phenotypic variability in plants, patterns of response to selection in plant populations, measurement of continuous and discontinuous genetic variability in plant populations.

Instructor

D. Wolyn
Rm. 4236 E.C. Bovey Building
Phone: ext. 53092
Email: dwolyn@uoguelph.ca

Course Objectives

The objective of this course is to familiarize students with basic concepts of plant genetics which include plant reproduction, genome organization, classical and molecular genetic analyses and population genetics. The university learning objectives addressed in this course include literacy, numeracy, understanding forms of enquiry, depth and breadth of understanding, independence of thought, and love of learning.

Course Content

I. Plant Reproduction
A. Sexual
  1. Gametogenesis
    a. angiosperms
    b. moss
B. Asexual

II. Natural pollination biology of angiosperms
A. Self-pollination
B. Cross-pollination
  1. Mechanisms
  2. Self-incompatibility
    a. Classical genetic analysis
    b. Molecular analyses

III. Plant Nuclear DNA Organization
A. Chromosomal
B. Molecular
C. Genome sequencing projects
D. Synteny

IV. Genetics of the Cytoplasm
A. Chloroplast
B. Mitochondria
  1. genome organization
  2. CMS: cytoplasmic male sterility

V. Analysis of Genetic Variation
A. Development and testing a single gene model
B. Chi-Square analysis
C. Test of heterogeniety

VI. Analysis of plant process through mutations
A. Methods
B. Tests of allelism
C. Dominant vs recessive
D. Epistasis

VII. Linkage
A. Chi-square analysis
B. Recombination and map distance
C . Assigning genes to chromosomes
  1. Classical method
  2. Molecular method
D. Maps
  1. Physical vs Genetic
  2. Molecular mapping
E. Map-based cloning

VIII. Polyploidy
A. Types
  1. Autoploids
  2. Alloploids
B. Genetics
  1. Meiosis
  2. Segregation

IX. Biotechnology
A. Gene transfer
B. Gene silencing
C. Forward vs. Reverse Genetics
  1. Segregation
  2. Transposon tagging
D. Genomics
  1. DNA chips
  2. Proteomics

Lecture & Lab Hours

Lecture: M, W, F, 9:30-10:20
Lab: Th, 9:30-11:20

Laboratory

The laboratory session will be used for review of special topics, data analysis and discussion of journal articles which highlight concepts of plant genetics. For journal article discussions, papers will be distributed one week in advance. All material covered in labs is relevant for quizzes and the final exam.

Tentative Schedule
1. No lab this week

2. No lab this week
3. Pollination Biology - Journal Article
4. Self-incompatibility - Journal Article
5. Organelles-Journal Article
6. Genetic Modelling - Data
7. Genetic Modelling - Journal Article
8. Mutant Screens - Journal Article
9. Linkage - Journal Article
10 Linkage - Journal Article
11. Polyploidy - Journal Article
12. Review

Problem Sets - Throughout the semester you will be given problems to reinforce or build upon lecture material. Solutions to problems will be discussed in lecture or lab, on a subsequent day. Students are expected to attempt solving problems and hand-in a copy of their best solution. Marks will be given for thoughtful attempts, not necessarily the correct solution.

Manuscript - In this exercise students will be given data related to the analysis of mutants for flowering time in Arabidopsis. Appropriate genetic analysis and statistical tests will be conducted. Results will be interpreted relative to current literature for flowering time and a short manuscript, suitable for publication, will be written.

Textbook

There is no one text for purchase. Topics will be covered from the following sources and will be available on reserve in the library and in the class reading binder in Dave’s office

1. Allard, R.W. Principles of Plant Breeding. 1965. John Wiley and Sons.

2. Briggs, F.N. and P.F. Knowles. Introduction to Plant Breeding. 1967. Reinhold Publishing.

3. Fehr, W.R. Principles of Cultivar Development. 1987. Macmillan Publishing

4. Griffiths, A.J.F., J.H. Miller, D.T. Suzuki, R.C. Lewontin and W.M. Gelbart. An Introduction to Genetic Analysis. 1996. W.H. Freeman.

5. Hughes, M.A. Plant Molecular Genetics. 1996. Addison Wesley Longman.

6. Koncz, C. N-H Chua and J. Schell. Methods in Arabidopsis Research. 1992. World Scientific.

7. Raven, P.H., R.F. Everet and SE Eichhrom. Biology of Plants. 1992. Worth Publishing.

8. Stansfield, W.D. Theory and Problems in Genetics. 1991. McGraw-Hill.

9. Wilson, C.L., W.E. Loomis and T.A. Steeves. Botany. 1971. Holt Rinehart and Winston.

10. Buchanan, B. B, W. Gruissem and R.L. Jones. Biochemistry and Molecular Biology of Plants. 2000. Am. Soc. Plant Physiologists.

Evaluation of Student Progress

Best 4 of 5 quizzes ............................................................. 40%
Problem Sets ....................................................................... 5%
Manuscript .......................................................................... 20%
Final exam .......................................................................... 35%